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Maximum image size: 3000 x 3000 pixels.
Supports any of the more than 150 image formats that meet the Bio-Formats standard.

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Number of total nuclei:
Number of IHC+ cells:
Percentage of IHC+ cells:
Click the button above to perform interactive size gating of the tumor cells and update the IHC scoring.

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Or process one of these sample images:

Sample Image
512 x 512  |  Ki67 Marker  |  20x
Image from BCDataset
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Image from DeepSlides
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Image from DeepSlides
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Image from DeepLIIF
Sample Image
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Image from DeepLIIF
Sample Image
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Image from DeepLIIF
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Image from DeepLIIF
Sample Image
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Image from neuroendocrine_

DeepLIIF is a virtual multiplex immunofluorescence (mpIF) restaining technique for immunohistochemistry (IHC) images. Given an IHC image input (brightfield Hematoxylin + DAB protein), DeepLIIF infers high-quality Hematoxylin (nuclear), mpIF DAPI (nuclear), mpIF LAP2Beta (nuclear envelop), mpIF protein marker, and semantic segmentation (blue/red cell segmentation masks for IHC scoring). DeepLIIF also generates high-quality mpIF modalities out-of-the-box for H&E images as well.

[Nature Machine Intelligence'22]

[CVPR'22]