Drag & drop your IHC image

or video for stitching here

(or click the upload button)

Upload your IHC image

or video for stitching

Maximum image size: 4096 x 4096 pixels.
For best results, use an image with resolution of 10X or higher.
Supports any of the more than 150 image formats that meet the Bio-Formats standard.
For video stitching, use an MP4 or AVI video less than 1 GB in size.
Do not upload images with protected health information (PHI) or personally identifiable information (PII).

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Please select the image resolution
and click begin to start processing.

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(Time can vary based on server load)

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Please select the video resolution
and click begin to start stitching.

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Number of total nuclei:
Number of IHC+ cells:
Percentage of IHC+ cells:
Click the button above to perform interactive size gating of the tumor cells and update the IHC scoring.

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Or process one of these sample images by clicking on it:

Sample Image
512 x 512  |  Ki67 Marker  |  40x
Image from BCDataset
Sample Image
512 x 512  |  Ki67 Marker  |  40x
Image from BCDataset
Sample Image
513 x 513  |  Ki67 Marker  |  40x
Image from DeepSlides
Sample Image
513 x 513  |  Ki67 Marker  |  40x
Image from DeepSlides
Sample Image
512 x 512  |  Ki67 Marker  |  40x
Image from DeepLIIF
Sample Image
512 x 512  |  Ki67 Marker  |  40x
Image from DeepLIIF
Sample Image
512 x 512  |  Ki67 Marker  |  40x
Image from DeepLIIF
Sample Image
512 x 512  |  Ki67 Marker  |  40x
Image from DeepLIIF
Sample Image
2100 x 1300  |  Ki67 Marker  |  20x
Image from neuroendocrine_
Sample Image
2100 x 1300  |  Ki67 Marker  |  20x
Image from neuroendocrine_
Sample Image
2202 x 2204  |  CD3 Marker  |  40x
Image from Pan-Tumor
Sample Image
2255 x 2246  |  CD3 Marker  |  40x
Image from Pan-Tumor
Sample Image
2212 x 2215  |  CD3 Marker  |  40x
Image from Pan-Tumor
Sample Image
2216 x 2215  |  CD3 Marker  |  40x
Image from Pan-Tumor

DeepLIIF is a virtual multiplex immunofluorescence (mpIF) restaining technique for immunohistochemistry (IHC) images. Given an IHC image input (brightfield Hematoxylin + DAB protein), DeepLIIF infers high-quality mpIF DAPI (nuclear), mpIF LAP2Beta (nuclear envelope), mpIF protein marker, and semantic segmentation (blue/red cell segmentation masks for IHC scoring). DeepLIIF also generates high-quality mpIF modalities out-of-the-box for H&E images as well.

[Nature Machine Intelligence'22]

[CVPR'22]

[MICCAI'23]

[Histopathology'23]

[MICCAI'24]